Design and engineer a fluorescence and luminescence microplate reader for high throughput analysis.
We deployed a cross-functional team to solve various optical, exterior design and prototyping challenges.
The first set of prototypes were built and tested in our facilities and the LEADseeker™ system was successfully launched as a fully automated plate reader capable of scanning and processing over a 1000 microplates per day.
At a glance
Team staff on project
A fluorescence and luminescence analyser
The brief was to design a fluorescence and luminescence reader system for high throughput analysis of high-density microplates, with robust, reliable optics and a simple user interface. The system had to support a broad application range and needed to work with multiple laboratory assays and user configurations.
We applied a systems approach to develop custom optics, electronics and mechanical modules. This enabled us to deliver a system capable of multiple excitation and emission wavelengths with even illumination from the excitation sources. We considered the manufacturing processes throughout the development ensuring the system could be produced and assembled efficiently by the eventual manufacturer.
Rapid, flexible imaging
To achieve the fast processing required, the system needed to image a whole micro-plate in one go. Imaging such a large area prevents having to index the plates accurately, but poses a significant challenge for conventional lens types: shadowing can occur in the wells furthest from the centre of the imaging field.
We decided to use a custom high-performance telecentric lens and a large footprint CCD. This allowed us to achieve high resolution imaging across the whole microplate.
A complex diagnostics system
The requirements of the device meant that we had to use a camera based around a back-illuminated Charged Coupled Device chip, which was designed to improve low-light performance and work with our client’s chemistry. This camera had to be mounted in a light-tight case and required cooling via an external refrigeration unit to minimise thermal noise.
To make the system entirely hands-free we automated the selection of both the emission and excitation filters. The filter automation allowed for a selection of six excitation wavelengths and four emission wavelengths. The plates themselves could be loaded automatically and the system was designed to be interfaced with automatic plate-loading robots.
We also improved the user interface. This included an easy plate loading and unloading solution housed in a sleek outer design.
In-house prototype build
While the formal transfer to volume manufacture took place, we built and tested a set of 20 prototypes in our Cambridge facilities. LEADseeker™ was able to automate production of high-resolution images and interrogate the luminescence and fluorescence of the samples.
The system enabled high-throughput analysis, imaging plates in seconds. The automation process allowed for hundreds of plates to be processed resulting in a significant asset in applications such as drug discovery and diagnostic screening.
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